Gene expression sequencing
De novo sequencing
Small RNA sequencing
Whole transcriptome sequencing
Areas of interest
Stem cell research
Ancient DNA genomics
Ion PI v3 Chip
~2.5 hours run time
Ion Proton System performance specifications with Ion PI Chi
ThroughputUp to 15 Gb
Read lengthUp to 200-base fragment readsNumber of reads passing filter60-80 million reads passing filterSequencing run time2.5 hoursBarcoding solutions384 barcodes supported by Torrent Suite Software
96 off-the-shelf barcodes for DNA
16 off-the-shelf barcodes for RNASoftware solutions
Torrent Suite Software utilizing Torrent Browser including TMAP alignment and Torrent Variant Caller for germline or somatic mutation detection.
Torrent Browser (included) offers users remote web access to instrument status monitoring, run quality reports, individual data files, and extensible plug-ins for application-specific analysis.
Plugins available for download from the Torrent Browser Plugin Store for analysis of genome sequencing, targeted sequencing, variant analysis and annotation, microbial sequencing, transfer to 3rd party bioinformatics packages, and more.
Data can optionally flow into Ion Reporter Software for controlled analysis, annotation, and reporting of variants. Ion Reporter Software provides application specific workflows to support single, trio and paired tumor normal analysis.
Data formatsIndustry standard FASTQ, SFF, BAM and VCF format outputs
Specifications for the Ion Proton Sequencer
Working environment (for indoor use only)Temperature: 68-77° F (20-25° C)
Humidity: 40-60%, noncondensing
Altitude: <6,500 ft (2,000 m)
12 in (30.5 cm) in rear
4 in (10 cm) on left side
4 in (10cm) on right side
4 in (10 cm) from front edge of bench to sequencer bezel
36 in (90 cm) aisle in front of bench for operator access
Optional rack mounting with two Ion Proton Sequencers per rack†Gas SupplyConnection: 0.25 in push-to-connect fitting
Pressure: 30 psi
Composition: nitrogen (grade 4.8, 99.998% or better)Other connectionsEthernet: 1 GigE
USB: 2x USB 2.0
PowerVoltage: 100 V (min) to 240 V (max)
Current: 14 A (max)
Frequencing: 50/60 Hz
Power Draw: 1,350 WDimensionsWidth: 21.3 in/54.2 cm
Depth: 30.5 in/77.5 cm
Height: 18.7in/47.4 cmWeightCrated for shipment: 200 lb/90.7 kg
Life Technologies Ion Torrent Ion Proton Semiconductor Sequencer. Condition is Used but in like new condition.